High-Level Gene Cassette Transcription Prevents Integrase Expression in Class 1 Integrons
نویسندگان
چکیده
منابع مشابه
Pc promoter from class 2 integrons and the cassette transcription pattern it evokes.
OBJECTIVES Integrons are considered expression systems due to the presence of Pc promoters that drive gene cassette transcription. The role and configurations of Pc are well known in class 1 integrons; however, this region has not yet been identified in class 2 integrons. This study aimed to characterize the Pc promoter from class 2 integrons and to determine the effect of gene cassette positio...
متن کاملDetection of gene cassettes in Tn402-like class 1 integrons.
Amplification of the gene cassettes in class 1 integrons by PCR using primers in the 5 conserved segment (5 -CS) and the 3 -CS (7, 8) has been used in hundreds of studies to identify integron-associated resistance genes (Fig. 1A). Equivalent PCR primers that detect cassettes in class 2 integrons, i.e., Tn7 family transposons (18), are also widely used. The approach used to detect integrons in a...
متن کاملGene cassettes and cassette arrays in mobile resistance integrons.
Gene cassettes are small mobile elements, consisting of little more than a single gene and recombination site, which are captured by larger elements called integrons. Several cassettes may be inserted into the same integron forming a tandem array. The discovery of integrons in the chromosome of many species has led to the identification of thousands of gene cassettes, mostly of unknown function...
متن کاملCostly Class-1 integrons and the domestication of the the functional integrase
Class-1 integrons play an important role in the emergence and spread of antimicrobial resistance determinants. In a recent study we showed that host fitness was dramatically reduced following acquisition of these elements. These fitness costs were due to the presence of an active integrase and we suggested that the mechanistic explanation was due to reduced genetic stability through IntI1 media...
متن کاملGene cassette arrays, antibiotic susceptibilities, and clinical characteristics of Acinetobacter baumannii bacteremic strains harboring class 1 integrons.
BACKGROUND AND PURPOSE Acinetobacter baumannii isolates containing class 1 integrons belong to different clones, but only a few strains are successful at causing infection. This study was conducted to compare the characteristics among these clones with different epidemicity. METHODS Eighty eight bacteremic isolates of A. baumannii were collected in a medical center in Taiwan during a 3-year p...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2011
ISSN: 0021-9193
DOI: 10.1128/jb.05246-11